On December 4, 2013, Society for Public Health Education, National Institutes for Health Office of Behavioral and Social Sciences Research and SAGE hosted a twitter chat exploring systems science applications in health promotion and public health. The twitter chat was based on a recently released supplement of Health Education & Behavior (HE&B) which examines the pioneering use systems science to address the complexity of public health research. In this chat, 10 questions were posed to authors and editors of the supplement about systems science in public health and about the research presented in the supplement. View the entire chat on Storify.